Figure 3.

PKM2 knockdown inhibits the migration and mobility of NCI-N87 cell. (A) Knockdown of PKM2 reversed aggressive morphological characteristics in NCI-N87 cells (100× magnification). (B and C) Silencing of PKM2 expression led to lower migration rate in NCI-N87 cells. Representative photomicrographs showing that at the 24 h time point migrated cells were present (200× magnification). The data represent the mean (±SD) from three independent experiments. (D and E) Wound healing assays were performed in the NCI-N87 cells with different expression levels of PKM2 to investigate the cell mobility. Representative image of scratches at 0 h, 24 h, 48 h, 72 h and 96 h were shown. The results of the wound healing assays were also shown as graphs. The data represent the mean (±SD) from three independent experiments (40× magnification). (F,G and H) Expression levels of N-cadherin, E-cadherin and Vimentin in stably transducted NCI-N78 cells were analyzed by western blot and qRT-PCR, quantitative western blot analysis results obtained using densitometric analysis and the mRNA expression levels which were standardized according to GAPDH. Full-length gels and blots are presented in the Supplementary files 2. The data represent the mean (±SD) from three independent experiments. **P < 0.01. ***P < 0.001. ****P < 0.0001.