Fig. 4.

Cell deposition rate (a); Hydrophobic partitioning (b); Zeta potential (c); QCM-D frequency change due to EPS adherence at 25 MHz (d); QCM-D frequency change due to EPS adherence at 35 MHz (e); and growth rates (f), for no cellulose, wild-type, and increased cellulose strains. Bacterial cell deposition was measured directly on a polypropylene membrane using a microscope-mounted flow cell in LBS media. Hydrophobic partitioning is based on relative affinity to n-dodecane of planktonic cells from cell suspension in 150 mM NaCl solution. Zeta potential was measured in 150 mM NaCl solution with OD (600 nm) of 0.1. Frequency change of QCM-D sensor is presented after 1 h exposure of 12.5 mg/l as TOC of extracted EPS in 0.34 M NaCl solution for sensor frequencies at either 25 MHz or 35 MHz. Cell growth was observed in LBS media by spectrophotometer at 25 °C. All error bars represent 1 standard error