Table 2.
Ratio of plasma and faecal bile acid content in relation to BL group.
| PLASMA | FAECES | |||
|---|---|---|---|---|
| HFD | HFD + P | HFD | HFD + P | |
| Total bile acids | 1.39 ± 0.56 | 1.07 ± 1.20 | 1.72 ± 0.34* | 2.30 ± 0.49***# |
| Conjugated bile acids | 1.56 ± 1.13 | 0.90 ± 0.57 | 2.07 ± 1.21 | 3.62 ± 2.96* |
| Unconjugated bile acids | 1.35 ± 0.48 | 1.11 ± 1.46 | 1.71 ± 0.33* | 2.27 ± 0.46***# |
| Individual bile acids | ||||
| Ursocholanic Acid | ND | ND | 1.16 ± 0.44 | 1.32 ± 0.47 |
| 3-Ketocholanic Acid | ND | ND | 1.12 ± 0.51 | 1.39 ± 0.70 |
| Lithocholic acid | ND | ND | 1.11 ± 0.33 | 1.25 ± 0.46 |
| Allolithocholic Acid | ND | ND | 1.38 ± 0.65 | 1.98 ± 1.08 |
| Isolithocholic Acid | ND | ND | 1.11 ± 0.55 | 1.22 ± 0.55 |
| 3,6/12-Diketocholanic Acid | ND | ND | 0.94 ± 0.80 | 1.58 ± 1.04 |
| 3α-Hydroxy-12 Ketolithocholic Acid | ND | ND | 1.74 ± 0.61 | 2.47 ± 0.87* |
| Deoxycholic Acid/Chenodeoxycholic | ND | ND | 1.29 ± 0.17 | 1.68 ± 0.28*p=0.052 |
| 5β-Cholanic Acid-3β, 12α-diol | ND | ND | 0.94 ± 0.23 | 1.17 ± 0.37 |
| Murocholic Acid | ND | ND | 3.07 ± 1.77** | 4.83 ± 2.54*** |
| Ursodeoxycholic acid | ND | ND | 1.58 ± 0.11* | 2.31 ± 0.36**## |
| Hyodeoxycholic acid | ND | ND | 2.26 ± 0.91** | 4.42 ± 1.91***# |
| 12-Dehydrocholic Acid | 0.59 ± 0.27 | 0.59 ± 0.78 | 2.41 ± 1.09* | 2.58 ± 1.29* |
| ω-Muricholic Acid | 0.93 ± 0.32 | 0.59 ± 0.77 | 1.87 ± 0.39** | 2.22 ± 0.46** |
| α/β-Muricholic acid | 2.41 ± 0.99 | 1.94 ± 2.49 | 2.98 ± 1.19*** | 4.28 ± 1.29*** |
| Hyocholic acid | ND | ND | 1.20 ± 0.38 | 1.67 ± 0.43 |
| Cholic acid | 1.49 ± 0.63 | 1.35 ± 1.82 | 17.00 ± 16.70 | 18.82 ± 15.33*** |
| Taurochenodeoxycholic Acid | 9.18 ± 9.09* | 4.36 ± 3.04 | 2.99 ± 1.32* | 8.40 ± 5.75***# |
| Taurodeoxycholic Acid | 1.08 ± 0.88 | 0.86 ± 0.53 | 6.12 ± 4.21** | 13.75 ± 13.81*** |
| Tauro-ursodeoxycholic Acid | 1.50 ± 0.26 | 1.16 ± 0.66 | 3.88 ± 2.51* | 6.25 ± 4.50** |
| Taurohyodeoxycholic Acid | ND | ND | 2.73 ± 1.50* | 7.25 ± 5.46** |
| Taurocholic Acid | 0.88 ± 0.82 | 0.50 ± 0.43 | 0.60 ± 0.22 | 1.29 ± 1.08 |
| Tauro-ω,α,β Muricholic Acid | 1.99 ± 1.27 | 1.04 ± 0.62 | 2.70 ± 1.76 | 3.64 ± 2.64** |
Data represent the means ± SD of 4 (plasma) or 6 (faeces) mice per group. Values of p were determined using one-way ANOVA with Tukey’s (equal variance) or Dunnett’s T3 (unequal variance) post-hoc analysis where ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 versus the BL group; # p < 0.05, ## p < 0.01, and ### p < 0.001 versus the HFD group. Values of p compared to the HFD group are stated where appropriate. ND, not detected.