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. 2017 Jun 6;7:2850. doi: 10.1038/s41598-017-03246-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The thickness and the viability of human iPS cell aggregations in the core-shell microfiber culture system. (A) Microscopic images showing the morphologies of human iPS cell aggregations in the core-shell microfiber culture and the suspension culture systems on day 4 and 8. Scale bar: 500 μm. (B) Thickness distributions of cell aggregations in the core-shell microfiber culture and the suspension culture systems on days 4 and 8. The thickness was calculated by measuring the minor axis of cell aggregation regarded as an ellipse. (C) The cell viabilities in the core-shell microfiber culture and the suspension culture systems after the retrieval of cell aggregations from the microfibers and dissociation of cell aggregations into single cells on days 4 and 8 (N ≥ 3). *P < 0.05.