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. 2017 Jun 6;7:2850. doi: 10.1038/s41598-017-03246-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Human iPS cells cultured in various types of core-shell microfibers. (A) Phase-contrast images showing the different types of human iPS cell-laden core-shell microfibers immediately after fabrication (day 0). Scale bar: 500 μm. (B) Merged phase-contrast and fluorescence images of the six types of core-shell microfibers cultured by day 4. Live cells were stained with calcein AM (green) and dead cells were stained with ethidium homodimer-1 (red). Scale bar: 500 μm. (C) Viability of the cells cultured in the six types of microfibers after the retrieval of cell aggregations from the microfibers and dissociation into single cells on day 4 (N ≥ 3).