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. 2017 Jun 6;7:2840. doi: 10.1038/s41598-017-02789-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Respiration of permeabilized cardiac fibers (J _O2, per wet weight), and flux control ratios normalized for NS-ETS capacity. (a) Oxygen consumption measured at 37 °C in SUIT 2 protocol: GM (N-LEAK respiration; GM_L), ADP (N-OXPHOS capacity; GM_P), cytochrome c (integrity of outer mt-membrane), pyruvate (N-OXPHOS capacity; PGM_P), succinate (NS-OXPHOS capacity; PGMS_P), FCCP (NS-ETS capacity; PGMS_E), rotenone (Rot; inhibition of CI, S-ETS capacity; S_E), malonic acid and antimycin A (Mna and Ama; less than 2% residual oxygen consumption, ROX). (b,c) ROX-corrected respiration at 37 °C and 4 °C: LEAK respiration and OXPHOS capacity with PM (SUIT 1, open boxes, n = 7 and 6 at 37 and 4 °C) or GM (SUIT 2, filled boxes, n = 16 and 8 at 37 and 4 °C). Note the switch of the flux control pattern for N-OXPHOS capacity (PM_P vs. GM_P). The two SUIT protocols merge at state PGM_P, but results are shown separately (not significantly different). Flux control ratios are normalized relative to median PGMS_E of the combined protocols. Box plots indicate the minimum, 25^th percentile, median, 75^th percentile, and maximum. *Significant differences between the two SUIT protocols for the same state. For abbreviations see Fig. 1.