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. 2017 Jun 6;8:929. doi: 10.3389/fpls.2017.00929

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Copyright © 2017 Pastore, Dal Santo, Zenoni, Movahed, Allegro, Valentini, Filippetti and Tornielli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Clustering analysis of the most differently expressed genes between Low (LT) and High (HT) growing temperature regime. Heat maps of the Fold Change (FC) of highly expressed (threshold value of raw fluorescence intensity ≥ 100) and modulated genes (|FC| ≥ 2) between LT and HT in at least one stage of development. Five clusters (A–E) were obtained by applying a K-Means Clustering analysis (KMC, Pearson’s correlation distance). A schematic representation of the general FC trend is depicted on top of each cluster heat map. Sample names are composed by temperature treatment abbreviation followed by the indication of the developmental stage (1, 2, 3 or 4). Data are the average of the three biological replicates.