Table 3.
Reproductive parameters of untreated female rats mated with male rats that received a diet of 5% of Jatropha curcas seed cake subjected to heat treatment and different methods of detoxification or standard feed (control) during 60 days.
| Parameters | Control | J. curcas nonhydrolyzed | J. curcas hydrolyzed | ||||
|---|---|---|---|---|---|---|---|
| I | Washed with ethanol (II) | Washed with water (III) | Unwashed (IV) | Washed with ethanol (V) | Washed with water (VI) | Unwashed (VII) | |
| Pregnant total | 9/10 | 8/10 | 8/10 | 10/10 | 9/10 | 8/10 | 9/10 |
| Maternal body weight (g)a | 360 ± 14.38 | 371.12 ± 11.31 | 377 ± 7.94 | 363.00 ± 8.03 | 351.33 ± 9.44 | 366.75 ± 14.15 | 369.44 ± 8.51 |
| Pregnant uterus weight (g)a | 61.57 ± 5.68 | 56.58 ± 5.25 | 62.01 ± 5.73 | 51.6 ± 6.24 | 61.10 ± 5.16 | 58.65 ± 6.61 | 61.89 ± 5.77 |
| Number of corpora luteaa | 13.66 ± 0.50 | 14.00 ± 0.60 | 13.25 ± 0.67 | 13.00 ± 0.65 | 13.77 ± 0.61 | 13.62 ± 0.56 | 13.55 ± 0.70 |
| Number of implantationsa | 13.33 ± 0.55 | 12.50 ± 0.59 | 12.25 ± 0.70 | 11.30 ± 0.65 | 12.44 ± 1.04 | 12.37 ± 0.56 | 12.66 ± 0.80 |
| Number of reabsorption sitesa | 0.44 ± 0.24 (3) | 0.87 ± 0.47 (4) | 0.50 ± 0.18 (4) | 1.90 ± 0.67 (8) | 0.88 ± 0.51 (3) | 0.75 ± 0.25 (5) | 0.77 ± 0.43 (3) |
| Placental weight (g)a | 0.40 ± 0.02 | 0.43 ± 0.04 | 0.44 ± 0.03 | 0.51 ± 0.03∗ | 0.46 ± 0.01 | 0.42 ± 0.02 | 0.45 ± 0.02 |
| Litter weight (g)a | 35.24 ± 4.89 | 34.29 ± 4.44 | 35.13 ± 4.56 | 29.03 ± 4.73 | 34.63 ± 4.27 | 35.72 ± 7.27 | 35.06 ± 5.45 |
| Body weight of fetuses (g)a | 2.70 ± 0.21 | 2.98 ± 0.18 | 2.91 ± 0.15 | 3.11 ± 0.25 | 2.94 ± 0.19 | 2.91 ± 0.36 | 2.92 ± 0.27 |
| Crown-rump length (cm)a | 4.90 ± 0.21 | 4.76 ± 0.12 | 4.75 ± 0.11 | 4.65 ± 0.16 | 4.88 ± 0.11 | 4.84 ± 0.30 | 4.90 ± 0.13 |
| Number of live fetusesa | 12.88 ± 0.73 | 11.62 ± 0.82 | 11.75 ± 0.79 | 9.20 ± 0.87∗ | 11.55 ± 0.83 | 11.62 ± 0.62 | 11.89 ± 0.69 |
| Number of dead fetusesa | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.20 ± 0.00 (1) | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 |
| Preimplantation loss (%)b | 2.45 ± 1.77 (2) | 10.30 ± 3.73 (5) | 7.36 ± 2.91 (5) | 11.97 ± 5.56 (4) | 10.57 ± 5.25 (4) | 8.85 ± 3.31 (5) | 6.53 ± 3.06 (4) |
| Postimplantation loss (%)b | 3.82 ± 2.14 (3) | 7.29 ± 4.00 (4) | 4.50 ± 1.75 (4) | 18.17 ± 6.11 (8) | 5.85 ± 3.30 (3) | 6.17 ± 1.94 (5) | 5.47 ± 2.94 (3) |
Data shown are mean ± SEM. Values in parentheses indicate the number of litters affected.
Where appropriate: aDunnett test; bKruskall-Wallis test. ∗P < 0.05 compared to the control group.