Figure 4.

PAE affected NF-κB canonical and ERK pathways. HSF cells were differentially cultured for 48 h, and the protein expression of (a) NF-κB canonical pathway and ERK pathway varied; (b) NF-κB canonical pathway proteins in the presence of BAY 11-7082; (c) NF-κB pathway expression in the presence of BAY 11-7082 and PAE. Actin was used as a loading control. Cells were incubated with a medium containing PAE at the indicated concentrations for 48 h after pretreatment with BAY 11-7082 (10 μM) for 30 min. Cell proliferation (d) was determined by MTT and (e) migration was tested by transwell assay. Data represent mean ± SD. ^∗p < 0.05; ^∗∗p < 0.01 versus control group.