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Immunofluorescence analysis of the spatial localization of the NF-κB canonical and ERK pathways. Cells were treated with PAE for 48 h, fixed, and incubated to determine the immunofluorescence. The antibodies against (a) RelA, (b) p-ERK, and (c) ERK presented green fluorescence, whereas a blue fluorescent signal was generated by DAPI staining of the cell nuclei. Areas of overlap between the green and the blue fluorescence appeared as merged images.
