Table 2.
The extraction recovery, precision, and accuracy of 20(R)-Rh1, 20(S)-Rh1, 20(R)-Rg3, and 20(S)-Rg3 in rat plasma (n = 5).
| Analytes | Spiked concentration (ng/mL) | Recovery (%) | RSD (%) | Intraday | Interday | ||
|---|---|---|---|---|---|---|---|
| Precision RSD (%) | Accuracy (%) | Precision RSD (%) | Accuracy (%) | ||||
| 20(R)-Rh1 | 1.0 | 81.0 | 9.6 | 3.9 | 105.7 | 5.3 | 104.2 |
| 10.0 | 82.4 | 2.3 | 3.3 | 96.2 | 4.0 | 101.3 | |
| 90.0 | 80.9 | 6.6 | 2.1 | 104.5 | 3.7 | 99.6 | |
|
| |||||||
| 20(S)-Rh1 | 1.0 | 85.1 | 7.9 | 5.6 | 94.9 | 11.7 | 95.0 |
| 10.0 | 81.3 | 3.7 | 5.2 | 105.4 | 5.9 | 106.1 | |
| 90.0 | 81.2 | 5.9 | 8.4 | 102.1 | 3.1 | 103.3 | |
|
| |||||||
| 20(R)-Rg3 | 1.0 | 83.3 | 9.2 | 7.2 | 109.5 | 6.7 | 107.6 |
| 10.0 | 70.4 | 3.1 | 4.4 | 103.9 | 6.1 | 102.6 | |
| 90.0 | 70.9 | 8.3 | 3.3 | 102.7 | 10.3 | 98.7 | |
|
| |||||||
| 20(S)-Rg3 | 1.0 | 83.8 | 2.0 | 2.5 | 89.2 | 8.7 | 92.4 |
| 10.0 | 71.7 | 2.1 | 7.1 | 96.7 | 7.5 | 95.9 | |
| 90.0 | 72.6 | 6.8 | 6.1 | 97.4 | 9.8 | 94.8 | |