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. 2017 Jun 5;36:75. doi: 10.1186/s13046-017-0545-x

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — AR promotes the anti-metastatic effect of ERβ1. a Wound-healing assay for the cell migration of ERβ1-expressing MDA-MB-231 and Hs578T cells with or without DHT (10nM) treatment. The percentage of wound healing was calculated. b Representative images of transwell assays for the invasion of MDA-MB-231 and Hs578T cells treated with or without DHT. c Western blot analysis of ZEB1, ERβ1, E-cadherin and N-cadherin protein levels in ERβ1-expressing MDA-MB-231 and Hs578T cells after treatment with or without DHT. d qRT-PCR detection of ERβ1 mRNA in ERβ1-expressing MDA-MB-231 and Hs578T cells with or without DHT treatment. *p < 0.05