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. 2017 Jun 5;18:442. doi: 10.1186/s12864-017-3827-y

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Bargraph of the number of detected genes across different protocols, degradation stages, and input amounts. The bar segments with the number of detected genes are listed by simplified Ensembl “Gene type” categories and the average number of detected genes per protocol is indicated by a black line. A gene is considered “expressed” if it has a FPKM value of at least 0.3 in one of the three technical replicates of at least one of the two samples (SEQC-A or SEQC-B)