Table 1.
List of pathogens detected using the high-throughput microfluidic real-time PCR amplification. The target gene refers to the gene used for the initial detection of the infectious agent and the confirmation method, the gene and procedure used for verifying the correction identification of the organism. NP: Not performed.
| Pathogens | Species | Target Gene (HT) | Positive ticks - Prevalence % (n = 201) | Confirmation method (gene targeted) | Reference confirmation technique | Confirmation | Sequence reference |
|---|---|---|---|---|---|---|---|
| Bacterium | Anaplasma spp. | msp2 | 3–1.5 | PCR (16S) | Hornok et al., 2008 | Anaplasma spp. | Sequence obtained without known species match |
| Bacterium | Bartonella henselae | pap31 | 1–0.5 | PCR (gltA) | Norman et al., 1995 | none | No sequence obtained |
| Bacteria | Borrelia spp. | 23s | 3–1.5 | Nested PCR (IGS and p66) | Bunikis et al., 2004 | none | No sequence obtained |
| Bacterium | Coxiella spp. | icd | 25–12.4 | NP | – | Coxiella-like symbiont | – |
| Bacterium | Francisella spp | tul4 | 3–1.5 | NP | – | Francisella like symbiont | – |
| Bacterium | Rickettsia helvetica | ITS | 6–3 | NP | – | R. helvetica | – |
| Bacteria | Rickettsia endosymbiont | gltA | 164–81.6 | PCR (gltA) | Regnery et al., 1991 | R. lusitaniae | gb|JQ771933.1 (Milhano et al., 2014) |
| Parasite | Babesia spp. | hsp70 | 6–3 | PCR (18S) | Bonnet et al., 2007 | Babesia spp. | Sequence obtained without known species match |
| Virus | West Nile (WNV) | polyprotein | 6–3 | RT-PCR (NS5) | Scaramozzino et al., 2001 | none | No sequence obtained |