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Effects of pH of mobile phases on the signal responses of HCAAs. HCAA standards (on-column amount of 10 pg) were applied for LC-MS/MS analysis. The gradient program was run as described in the experimental section. Four types of mobile phase A (5 mM ammonium formate at pH 3.8, 5.7, 8.8 and 0.05% NH4OH in H2O at pH 10.5) were tested for best signal responses of HCAAs.
