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Representative MRM chromatograms of total urinary HCAAs detected in smokers with AαC at 69.8 pg/mL, MeAαC at 4.58 pg/mL, PhIP at 3.35 pg/mL, Harman at 774 pg/mL, Norharman at 371 pg/mL. The urine samples were hydrolyzed at 70 °C for 5 hours with basic condition and prepared by robotic sample preparation system prior to LC-MS/MS analysis.
