Table 1. Compound dependent MS parameters and ion transitions for HCAA analysis.^a.

		Precursor ions (m/z)	Product ions (m/z)	DPb (V)	EPc (V)	CEd (V)	CXPe (V)
Harman	Quantitation ion	183.1	115.0	260	10	68	14
	Confirmation ion	183.1	88.9	260	10	63	14
	Internal standard ion	186.1	117.1	260	10	68	14
Norharman	Quantitation ion	169.1	115.1	170	10	82	14
	Confirmation ion	169.1	88.9	170	10	105	10
	Internal standard ion	176.2	120.0	170	10	82	14
AαC	Quantitation ion	184.1	167.1	51	10	31	22
	Confirmation ion	184.1	140.0	51	10	43	20
	Internal standard ion	187.0	169.1	51	10	31	22
MeAαC	Quantitation ion	198.1	181.1	36	10	31	16
	Confirmation ion	198.1	127.1	36	10	53	12
	Internal standard ion	201.1	184.1	36	10	31	16
IQ	Quantitation ion	199.1	184.1	56	10	35	18
	Confirmation ion	199.1	157.1	56	10	47	12
	Internal standard ion	202.2	184.2	56	10	35	18
Trp-P-1	Quantitation ion	212.1	167.1	41	10	55	16
	Confirmation ion	212.1	195.0	41	10	32	16
	Internal standard ion	215.1	168.0	41	10	55	16
Trp-P-2	Quantitation ion	198.1	181.1	36	10	31	16
	Confirmation ion	198.1	127.0	36	10	53	12
	Internal standard ion	201.1	183.1	36	10	31	16
Glu-P-1	Quantitation ion	199.1	92.0	121	10	45	12
	Confirmation ion	199.1	172.1	121	10	34	12
	Internal standard ion	202.0	92.0	121	10	45	12
Glu-P-2	Quantitation ion	185.1	168.1	126	10	35	10
	Confirmation ion	185.1	78.0	126	10	49	10
	Internal standard ion	188.1	171.1	126	10	35	10
PhIP	Quantitation ion	225.1	210.1	111	10	39	28
	Confirmation ion	225.1	115.0	111	10	61	10
	Internal standard ion	228.2	210.1	111	10	39	28

^aParameters for Harman and Norharman have been detuned to avoid saturating detector.

^bDeclustering potential.

^cEntrance potential.

^dCollision energy.

^eCollision cell exit potential.