. Author manuscript; available in PMC: 2017 Nov 1.

Published in final edited form as: Anal Bioanal Chem. 2016 Sep 29;408(28):8149–8161. doi: 10.1007/s00216-016-9917-x

Table 2. Minimal matrix effects on calibration curves of HCAA prepared in urine.

	Water calibration curves on-column (pg)	Urine calibration curves (pg/ml)	IS conc. in urine (pg/ml)	Calibration curves	R²	Difference in slopes %
AαC	0.098-19.7	0.98-197	50	Water: y=0.984x+0.00167	0.999	2.6
				Urine: y=1.01x+0.00394

MeAαC	0.1-20	1-200	50	Water: y=0.857x+0.000561	0.999	1.7
				Urine: y=0.872x+0.000126

IQ	0.1-20	1-200	30	Water: y=1.24x+0.00386	0.999	0.8
				Urine: y=1.25x+0.00787

Trp-P-1	0.078-15.6	0.78-156	39	Water: y=0.984x+0.00153	0.999	0.5
				Urine: y=0.989x+0.000628

Trp-P-2	0.067-13.5	0.67-135	38.4	Water: y=0.923x+0.00158	1.000	3.3
				Urine: y=0.954x+0.0012

Glu-P-1	0.073-14.7	0.73-147	78.4	Water: y=1.22x+0.00152	1.000	0.81
				Urine: y=1.23x+0.00282

Glu-P-2	0.066-13.3	0.66-133	107.4	Water: y=1.10x+0.00035	1.000	0.92
				Urine: y=1.09x+0.00428

PhIP	0.1-20	1-200	25	Water: y=1.04x+0.0216	0.998	2.8
				Urine: y=1.07x+0.00116

Harman	1-400	10 -4000	300	Water: y=1.14 x+0.00458	1.000	2.6
				Urine: y=1.17x+4.05

Norharman	1-400	10 -4000	300	Water: y=1.43x+0.00875	0.999	0.7
				Urine: y=1.42x+2.03