Table 3. Intra-day, inter-day precision and accuracy, LOD, LOQ, recoveries, ion suppression for HCAA analysis.

Analyte		Intraday						Interday
	Nominal Concentration (pg/mL)	Determined Concentration (pg/mL)			Accuracy (%)	RSD (%)	Nominal Concentration (pg/mL)	Determined Concentration (pg/mL)			Accuracy (%)	RSD (%)	LOQ (pg/mL)	LOD (pg/mL)	Recovery (%)	Ion suppression (%)
		n=8						n=6
AαC	150	151	±	2.3	101	1.5	150	148	±	5.7	98	3.9	2.1	0.62	64	47
	40	41	±	1.6	103	4	40	40	±	1.8	100	4.5
	10	10	±	0.2	101	1.7	10	10	±	0.5	100	5
MeAαC	150	150	±	1.1	100	0.8	150	144	±	6.9	96	4.8	1.1	0.33	54	49
	40	40	±	0.8	101	2	40	38	±	2.1	96	5.5
	10	10	±	0.1	98	0.8	10	10	±	0.4	95	4.2
IQ	150	152	±	1.8	102	1.2	150	152	±	11.2	102	7.3	2	0.37	75	26
	40	42	±	1	105	2.4	40	41	±	3.2	103	7.7
	10	11	±	0.3	106	3.2	10	10	±	0.8	102	7.8
Trp-P-1	117	117	±	2.1	100	1.8	117	112	±	4.9	96	4.3	2.6	0.79	93	39
	31	32	±	1.8	101	5.7	31	30	±	1.7	96	5.7
	7.8	7.5	±	0.2	97	3.2	7.8	7.2	±	0.7	93	9.9
Trp-P-2	101	104	±	2.4	103	2.3	101	100	±	4.1	99	4.1	1.2	0.63	90	38
	27	28	±	1.2	104	4.3	27	26	±	1.5	98	5.8
	6.7	6.6	±	0.2	98	2.8	6.7	6.4	±	0.4	95	6.3
Glu-P-1	105	104	±	0.8	98	0.8	105	104	±	1.4	99	1.3	1	0.31	44	15
	28	28	±	0.2	100	0.8	28	28	±	0.4	98	1.4
	7	7	±	0.2	100	2.7	7	6.8	±	0.2	98	2.3
Glu-P-2	107	106	±	0.8	99	0.7	107	105	±	2.1	98	2	2.8	0.83	55	24
	29	29	±	0.2	100	0.8	29	28	±	0.7	97	2.6
	7.2	7.4	±	0.3	103	4.1	7.2	7.1	±	0.3	99	4.2
PhIP	150	149	±	1.8	100	1.2	150	147	±	3.9	98	2.7	1.1	0.34	79	21
	40	40	±	0.5	99	1.3	40	39	±	1	97	2.6
	10	10	±	0.2	100	2	10	10	±	0.4	96	4.2
		Intraday						Interday
		n=4						n=3
Harman	3530	3825	±	205.3	108	5.4	3530	3583	±	302.8	102	8.5			93	28
	313	335	±	18.1	107	5.4	313	341	±	32.6	109	9.6
NorHarman	2132	2120	±	37.4	99	1.8	2132	2171	±	118.9	102	5.5			86	24
	662	690	±	27.3	104	4	662	710	±	63	107	8.9
Harman a	4000	3816	±	205.2	95	5.4	4000	3832	±	176.8	96	4.6	15.3	4.6
	400	401	±	6.9	100	1.7	400	389	±	17.5	97	4.5
	100	100	±	2	100	2	100	97	±	5.5	97	5.7
NorHarman a	4000	4024	±	96	101	2.4	4000	4073	±	137	102	3.4	42	12.6
	400	416	±	3.6	104	0.9	400	418	±	16.8	104	4
	100	105	±	1.3	105	1.2	100	104	±	5.1	104	4.9

^aPrepared in synthetic urine.