Figure 1.

Floxing the Pgrmc2 allele. (A) Schematic of conditional mutagenesis approach used to ablate floxed regions of the Pgrmc1 and Pgrmc2 genes. (B) A PCR-based genotyping protocol was developed to identify control (Pgrmc2^fl/fl), heterozygous (Pgrmc2^fl/d), and conditional knockout (Pgrmc2^d/d) mice. (C) qPCR showing that Pgrmc2 expression is reduced in uterine tissue isolated from Pgrmc2^d/d female mice compared with tissue obtained from Pgrmc2^fl/fl mice. Pgrmc2 expression does not change in the liver regardless of genotype (*P < 0.05, n = 3). L, ladder.