Insulin prevents systemic hypertension and renal oxidative stress in Akita
mice. (a) Longitudinal changes in mean SBP (measured 2 to 3 times per mouse per
week in the morning without fasting). Baseline SBP was recorded daily over 5
days before initiation of measurements. (b) Cat immunostaining, (c)
semiquantitation of Cat-immunostained areas, (d) Cat activity, (e)
Cat mRNA level, (f) DHE (red) staining (left panel) and
semiquantitation of DHE fluorescence (right panel), (g) ROS generation by
lucigenin assay, (h) NADPH oxidase activity, (i) Nox4, (j)
Nox1, and (k) Nox2 mRNA expression in
freshly isolated RPTs from WT controls, Akita mice, and Akita mice + insulin
(Ins) implants. Values are mean ± SEM, n = 8 per group.
*P < 0.05; **P
< 0.01, and ***P < 0.005,
WT vs Akita. ††P < 0.01, Akita
vs Akita-Ins. WT controls (open bars); Akita (solid bars), and Akita mice + Ins
(gray bars). DAPI, 4′, 6-diamidino-2-phenylindole; NS, not significant;
RLU, relative luciferase unit.