Insulin effect on Nrf2, Agt, hnRNP F, and hnRNP
K gene promoter activity in IRPTCs. Cells stably transfected with
(a) pGL4.20-Nrf2, (b) pGL4.20-Agt, (c)
pGL4.20-hnRNP F, or (d) pGL4.20-hnRNP K
gene promoter were incubated in NG or HG DMEM ± insulin for 24 hours
with or without wortmannin, Ly-294, 002, PD98059, or U0126 or transiently
transfected with p42 MAPK or p44 MAPK siRNA (e–h). Luciferase activity
in cells cultured in NG medium was considered as 100%. The results are
expressed as percentage of control (mean ± SEM, n = 3).
*P < 0.05; **P
< 0.01; ***P < 0.005.
Similar results were obtained in two separate experiments. NS, not significant;
RLU, relative luciferase unit.