Oltipraz effect on Agt, Nrf2, hnRNP F, and hnRNP
K gene expression in IRPTCs. Effect of oltipraz on promoter
activity of (a) Nrf2, (b) Agt, (c)
hnRNP F, and (d) hnRNP K genes and their
respective mRNA (e–h) and protein (i and j) levels in IRPTCs incubated
in NG or HG medium ± insulin with or without PD98059. Promoter activity,
mRNA, and protein levels in cells in NG medium are considered as 100% or
arbitrary unit 1, respectively. The results are reported as percentages of
control values (mean ± SEM, n = 3). *P <
0.05; **P < 0.01;
***P < 0.005. Similar results
were obtained in three separate experiments. Control IRPTCs in NG (open bars),
IRPTCs treated with oltipraz (solid black bars), IRPTCs treated with oltipraz +
Ins (horizontal striped bars), and IRPTCs treated with oltipraz + insulin +
PD98059 (diagonal striped bars). DMSO, dimethyl sulfoxide; NS, not significant;
RLU, relative luciferase unit.