Figure 2.
Reduced pituitary IGSF1 protein and mRNA expression in Igsf1Δ312/y mice. (a) Proteins extracted from four adult wild-type (lanes 1 to 4) and six Δ312 males (lanes 5 to 10) were subjected to SDS-PAGE and immunoblotting using an anti–IGSF1-CTD antibody. Arrows mark the mature and immature glycoforms in wild-type and the truncated protein in Δ312 males. *, nonspecific bands. (b) Protein extracts from two wild-type (lanes 1 to 6) and two Δ312 males (lanes 7 to 12) were treated with EndoH or PNGaseF prior to analysis as in (a). (c) RNA was extracted from pituitaries of adult wild-type (circles) or Δ312 males (squares). mRNA expression of Igsf1 was analyzed by reverse transcription qPCR using primers common to both transcripts. Group means are indicated with solid horizontal lines. The data were analyzed by a two-tailed t test (t = 19.95, df = 12.64, ****P < 0.0001). IB, immunoblotting; WT, wild-type.