Figure 5.
Thyroid hormone feedback mechanisms appear to be intact in Igsf1-deficient mice. (a) Representative ISH images of Trh mRNA expression in the PVN of brains from 8-week-old Igsf1+/y and Igsf1Δ312/y mice on a normal diet. (b) Quantification of average Trh ISH signal in Igsf1+/y and Igsf1Δ312/y brains. The data were analyzed by two-tailed t test: t = 5.861, df = 21.87, ****P < 0.0001. (c) Representative ISH images of Trh mRNA expression in the PVN of brains from 11-week-old Igsf1+/y and Igsf1Δex1/y mice on a LoI/PTU diet for 3 weeks. On the last 4 days of the experiment the mice were injected with one of four treatments (saline, low T3, high T3, high T4). (d) Quantification of average Trh ISH signal in Igsf1+/y and Igsf1Δex1/y brains in each condition. The data were analyzed by two-way ANOVA [interaction: F(3, 19) = 0.4753, N.S. P = 0.7032; treatment: F(3, 19) = 72.83, ****P < 0.0001; genotype: F(1, 19) = 0.02785, N.S. P = 0.8692]. N.S., not significant.