Figure 6.
TRH-induced TSH release is blunted in Igsf1-deficient mice. (a) Eight-week-old male wild-type and Igsf1Δ312/y mice were injected with TRH (i.p. 10 μg/kg). Blood samples were collected before the TRH injection as well as 15 minutes, 1 hour, and 2 hours after injection. Plasma TSH levels were measured by ELISA. Data were analyzed by two-way ANOVA [interaction: F(3, 99) = 30.31, ****P < 0.0001; time after injection: F(3, 99) = 160.9, ****P < 0.0001; genotype: F(1, 99) = 37.67, ****P < 0.0001] and post hoc t test, n = 13 for each genotype. The data from the 15-minute time point were expanded to show individual data points (two-tailed t test: t = 5.627, df = 22.72, ****P < 0.0001). (b) Male 8-week-old wild-type and Igsf1Δex1/y mice were injected with TRH (i.p. 10 μg/kg). Blood samples were collected prior to and 15 minutes after TRH injection. Serum TSH levels were measured by RIA. Data were analyzed by two-way ANOVA [interaction: F(1, 20) = 11.01, **P = 0.0034; time: F(1, 20) = 79.65, ****P < 0.0001; genotype: F(1, 20) = 13.88, **P = 0.0013] and post hoc t test (t = 3.531, df = 8.466, **P = 0.0071), n = 6 per genotype.