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. 2017 Feb 1;158(4):1022–1033. doi: 10.1210/en.2016-1558

Figure 1.

Figure 1.

X. tropicalis HAL2 is strongly upregulated by T3 at the climax of intestinal metamorphosis when adult stem cells are forming and proliferating. (a) HAL2 expression peaks at the climax of natural metamorphosis. Total RNA was isolated from whole intestine of tadpoles at premetamorphosis (stage 54), prometamorphosis (stages 56, 58), climax (stages 61, 64), and at the end of metamorphosis (stage 66) and subjected to qRT-PCR analysis for HAL2 expression. Note that HAL2 levels were high at the climax. (b) HAL2 expression is induced by T3 treatment of premetamorphic tadpoles. Premetamorphic stage 54 tadpoles were treated with 10 nM T3 for 2 days. Total RNA was isolated from the whole intestine and subjected to qRT-PCR analysis for HAL2 expression.