Table 1.
Antibodies Used for Different Experiments in This Report
| Antibody | Host Species | Vendor | Catalog Number |
Working Dilution |
|
|---|---|---|---|---|---|
| IB | IF/IHC | ||||
| Actin | Goat | Santa Cruz Biotechnology | sc-1616 | 1:300 | |
| Akt | Rabbit | Cell Signaling Technology | 9272 | 1:1000 | |
| p-Akt1 S473 | Rabbit | Cell Signaling Technology | 4060 | 1:1000 | |
| p-Akt2 S474 | Rabbit | Cell Signaling Technology | 8599 | 1:1000 | |
| Arp3 | Mouse | Sigma-Aldrich | A5979 | 1:3000 | 1:50 |
| α-tubulin | Mouse | Abcam | ab7291 | 1:1000 | 1:300 |
| β-catenin | Rabbit | Thermo Fisher Scientific | 71-2700 | 1:250 | 1:100 |
| β-tubulin | Rabbit | Abcam | ab6046 | 1:1000 | |
| CAR | Rabbit | Santa Cruz Biotechnology | sc-15405 | 1:200 | 1:50 |
| Detyrosinated α-tubulin | Rabbit | Abcam | ab48389 | 1:1000 | |
| Dia1 | Goat | Santa Cruz Biotechnology | sc-10885 | 1:200 | |
| EB1 | Mouse | BD Biosciences | 610534 | 1:200 | |
| EB1 | Rabbit | Santa Cruz Biotechnology | sc-15347 | 1:200 | |
| Eps8 | Mouse | BD Biosciences | 610143 | 1:5000 | 1:50 |
| Laminin α2 | Mouse | Millipore | MAB1922 | 1:300 | 1:300 (tissue) 1:100 (cell) |
| MARK2 | Rabbit | Proteintech | 15492-1-AP | 1:2000 | |
| MARK4 | Rabbit | Proteintech | 20174-1-AP | 1:2000 | |
| mTOR | Rabbit | Cell Signaling Technology | 2983 | 1:2000 | |
| N-cadherin | Mouse | Thermo Fisher Scientific | 33-3900 | 1:200 | 1:100 |
| Raptor | Rabbit | Cell Signaling Technology | 2280 | 1:1000 | |
| rpS6 | Rabbit | Cell Signaling Technology | 2217 | 1:1000 | |
| p-rpS6 S235/236 | Rabbit | Cell Signaling Technology | 4858 | 1:1000 | |
| p-rpS6 S240/244 | Rabbit | Cell Signaling Technology | 5364 | 1:1000 | |
| ZO-1 | Rabbit | Thermo Fisher Scientific | 61-7300 | 1:250 | 1:100 |
| Goat IgG-HRP | Bovine | Santa Cruz Biotechnology | sc-2350 | 1:3000 | |
| Rabbit IgG-HRP | Bovine | Santa Cruz Biotechnology | sc-2370 | 1:3000 | |
| Mouse IgG-HRP | Bovine | Santa Cruz Biotechnology | sc-2371 | 1:3000 | |
| Rabbit IgG-Alexa Fluor 555 | Goat | Thermo Fisher Scientific | A-21429 | 1:250 | |
| Mouse IgG-Alexa Fluor 488 | Goat | Thermo Fisher Scientific | A-11029 | 1:250 | |
| Mouse IgG-Alexa Fluor 555 | Goat | Thermo Fisher Scientific | A21422 | 1:250 | |
Abcam, Cambridge, MA; Cell Signaling Technology, Danvers, MA; Santa Cruz Biotechnology, Santa Cruz, CA; Sigma-Aldrich, St. Louis, MO; Invitrogen, Life Technologies, Carlsbad, CA; Proteintech, Chicago, IL; BD Biosciences, San Jose, CA; Millipore Corp., Billerica, MA.
Abbreviations: Arp3, actin-related protein 3; EB1, end-binding protein 1; Eps8, epidermal growth factor receptor pathway substrate 8; IHC, immunohistochemistry; MARK, microtubule affinity-regulating kinase.