Figure 6.

PI3K activation is involved in P4 and nPR signaling in female rat ventricular myocytes. (a) Representative immunoblot and quantification of PLN17 phosphorylation and total PLN under control (Cont), BPA, and BPA in the presence of P4, P4 + 1 μm wortmannin (Wort), or Wort. (b) Representative recordings and percentages of myocytes with triggered activities under various treatments (n = 32 to 46 myocytes from four hearts). (c) Representative immunoblot and quantification of Akt473 phosphorylation and total Akt under control, P4, and P4 in the presence of RU486, PTX, or Wort. For all experiments, the concentrations of treatments are as follows: BPA and P4 = 1 nM; RU486 and Wort = 1 μM; PTX = 200 ng/mL. **P < 0.01; ^#P > 0.1 versus control; ^†P < 0.01 (χ² test).