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. 2017 Jan 24;158(4):920–935. doi: 10.1210/en.2016-1538

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This article has been published under the terms of the Creative Commons Attribution License (CC BY; https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Copyright for this article is retained by the author(s).

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Figure 4. — Follicles cultured in the presence of DHT had a 10-fold increased incidence of early antrum formation. (a) The number of follicles in the absence and presence of DHT that formed an antrum by 72 hours of culture. Numbers above the bars are the percentage of follicles that formed an antrum (P < 0.0001; two-sided Fisher’s exact test. (b–f) Examples of preantral follicles with an antrum (arrowed, translucent area within the GCs) cultured in DHT. (g) Confocal image of DHT-treated follicle exposed to DHT in vitro with oocyte (ooc) and antrum arrowed. (h) Diameter of follicles at 48 hours (i.e., at the onset of possible antrum formation) that did, or did not, have an antrum at 72 hours. Four statistical comparisons were made (to compare diameter of follicles with and without antra within a treatment; and of follicles with, or without, antra between treatments) using one-way ANOVA with a Sidak’s multiple comparison test. ***P < 0.001. ns, not significant.