Figure 5.

Follicles cultured in the presence of DHT had a greater proportion of Ki67-positive (green) nuclei than in control conditions after 24 hours (a) and 72 hours (b) of culture. Bars are means and 95% confidence interval; treatments were compared using an unpaired t test. **P < 0.01. (c and d) Confocal images of Ki-67–positive nuclei (green); scale bars = 50 µm. (e–h) There was no significant effect of treatment on TUNEL (e and f, green) and caspase (g and h, red) expression in control and DHT-treated follicles at 24 and 72 hours. Bars are medians; treatments were compared using a Mann-Whitney test. (i–g) Confocal images of TUNEL-labeled (green) and active caspase-positive GCs (red) in follicles at 24 hours (i and j) and 72 hours (k–n) cultured in the absence (i, k, and m) and presence (j, l, and n) of DHT. Nuclei were counterstained with DAPI (blue). Scale bars = 25 µm (i–n).