Fig 3. Th1 differentiation of CD4+ T lymphocytes induced by K. pneumoniae strains.
PBMC from 6 healthy donors were cultured at 106/ml for 7 days with UV-inactivated bacterial cells at 1:10 ratio. Cells were recovered at the indicated times, stained with anti-CD4-APC and anti-IFNγ-PerCP and analyzed by ACCURI instrument using the CflowPlus software to process the data. The area of positivity was determined by using an isotype-matched control mAb. Representative scatter plots at day 5 are shown. Data are expressed as percentage of CD4+IFNγ+ (mean ± SE).