Figure 10.

Adipocyte-specific inactivation of Hilpda has no effect on tissue weights or plasma parameters following a 24-hour fast. (a) Hilpda mRNA (left) and HILPDA protein levels (right) in eWAT from C57BL/6 mice fed or fasted for 18 hours (n = 6/group). Gene expression levels of fed mice were set at one. (b) Weight of eWAT of male Hilpda^flox/flox (n = 7) and Hilpda^ΔAT (n = 8) fasted for 24 hours. (c) Plasma levels of NEFA, glycerol, triglycerides, glucose, and ketone bodies of male Hilpda^flox/flox (n = 7) and Hilpda^ΔAT (n = 8) mice fasted for 24 hours. (d) Plasma levels of insulin and adiponectin of Hilpda^flox/flox (n = 7) and Hilpda^ΔAT (n = 8) mice fasted for 24 hours. (e) Triglyceride (TG) levels in livers of Hilpda^flox/flox (n = 7) and Hilpda^ΔAT (n = 8) mice fasted for 24 hours. (f) mRNA levels of genes involved in lipolysis or lipid droplet morphology (Pnpla2, Lipe, Abhd5, G0S2, Plin1, Plin2, Plin4) in eWAT of Hilpda^flox/flox (n = 7) and Hilpda^ΔAT (n = 8) mice fasted for 24 hours. Gene expression levels for Hilpda^flox/flox mice were set at one for each gene. Data are mean ± standard error of the mean. Asterisks indicate significant differences according to Student t test relative to (a) fed mice or (b–e) Hilpda^flox/flox mice ; **P < 0.01; *P < 0.05.