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. 2017 Jun 6;12(6):e0177761. doi: 10.1371/journal.pone.0177761

Fig 2. Characterization of EGFR-NLP size and shape.

Fig 2

A, Following NLP self-assembly, the specified NLPs were separated by a Yarra 3u SEC-4000 column and typical traces are shown. Empty NLPs elute between 9.5–10.5 minutes (P2) with EGFR-NLPs eluting earlier at 5.5–9.5 minutes (P1). Numbers above indicate molecular mass (in kDa) of known protein standards. B, Quantification of area under the curve of the P1 and P2 SEC peaks in μV*sec from a typical SEC trace. C, Empty and EGFR-NLPs were analyzed by DLS to determine NLP diameter.