Fig 4. NLP-associated EGFR is capable of binding its ligand, EGF.

A, Schematic of the OctetRed assay used to determine EGFR ligand binding. Streptavidin biosensors were loaded with biotinylated EGF and EGFR-NLP association and dissociation with the sensor was quantitated via BLI. B, Association (0–2000 s) and dissociation (2000–4000 s) curves of EGFR-NLPs binding to biotinylated EGF on the sensors. EGFR-NLP concentrations were titrated by a 1.5-fold dilution to the concentrations displayed to the right of the graph. Curves are normalized by subtracting the 0 μM EGFR-NLP curve. C, Log plot of EGFR-NLP binding response (nm) to biotinylated EGF. Plot shows mean of two technical duplicates and are representative of results from three biological repeats.