Fig 5. NLP assemblies contain EGFR monomers, dimers, and multimers.

Empty and EGFR-NLPs were separated by SDS-PAGE with (+β-ME/heat) and without denaturing conditions (-β-ME/heat) to observe EGFR multimers and analyzed by immunoblotting with anti-pY4G10, anti-EGFR, and anti-ApoA1 antibodies. Similar results were observed in each of three biological replicates. Numbers indicate band intensity relative to EGFR-NLPs normalized to ApoA1 signal.