Table 2. Main differential phenotypic characteristics of the studied M. pachydermatis strains.
Tween diffusion test | ||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Strain | Growth on SGA | Growth on mDA | Growth at 42°C | beta–glucosidase activity | with SGA | with YNBA | ||||||||
T 20 | T 40 | T 60 | T 80 | CrEL | T 20 | T 40 | T 60 | T 80 | CrEL | |||||
CBS 1879 | + | +1 | w | - | + | + | + | + | + | +a | + | + | +a | - |
CBS 1884 | + | +1 | w | - | +b | + | + | + | + | +a | + | + | - | - |
CBS 6535 | + | +1 | w | - | + | + | + | + | + | +b | + | + | +a | - |
MA-13 | + | +1 | w | - | v | +b | + | + | +b | +b | + | + | w | w |
MA-52 | + | +1 | w | - | - | + | + | + | +b | +a | + | + | w | w |
MA-56 | + | +1 | + | - | - | + | + | + | +b | w | + | + | +a | +a |
MA-94 | + | +1 | + | - | - | + | + | + | +b | w | + | + | +a | - |
MA-107 | + | +2 | - | - | + | + | + | + | +b | w | + | + | +a | w |
MA-140 | + | +1 | + | - | - | +b | + | + | +b | +b | + | + | +a | +a |
MA-195 | + | +1 | + | - | v | +b | + | + | +b | +a | + | + | w | w |
MA-280 | + | +2 | w | - | + | + | + | + | +b | +b | + | + | w | - |
MA-312 | + | +1 | w | - | +b | + | + | + | + | +b | + | + | + | - |
MA-356 | + | +1 | w | - | v | +b | + | + | +b | + | + | + | +a | - |
MA-366 | - | +1 | - | + | +a | + | + | + | w | + | + | + | + | w |
MA-374 | - | +1 | - | + | +a | + | + | + | w | + | + | + | + | w |
MA-380 | - | +1 | - | + | +a | + | + | + | w | + | + | + | + | w |
MA-475 | + | +1 | + | - | - | +b | + | + | +b | +b | +b | + | +a | - |
MA-579 | + | +2 | - | - | w | + | + | + | + | +a | + | + | w | +a |
MA-1382 | + | +1 | w | - | w | +b | + | + | +b | + | + | + | - | - |
Growth on SGA (Sabouraud Glucose Agar) at 32°C; Growth on mDA (modified Dixon Agar) after 7 days of incubation at 32°C: +1 colony diameter of 2–5 mm
+2 colony diameter of <1 mm; Growth at 42°C on mDA after 7 days of incubation.
Tween diffusion test [1, 15] with SGA and YNBA (Yeast nitrogen base agar): +, good growth; w, weak growth; +a, growth at a distance of the well where the substrate was placed; +b, ring of growth inhibition at a distance of the well; -, growth inhibition; v, variable results between replicates.
Naming source: CBS, Centraalbureau voor Schimmelcultures; MA, culture collection of the Veterinary Mycology group.