Fig 1. Ply activity and expression.

Effects of potD deletion on hemolytic activity were assessed via hemolysis assay, in which pneumococcal lysates were incubated with sheep RBCs and lysis was monitored. Lysis by Triton X-114 was set at 100%. Effects on Ply synthesis were determined using a standard indirect ELISA, in which WT values were set at 100%. Deletion of potD from MNZ67 significantly reduced hemolytic potential (A). A significant reduction in Ply production was also observed upon deletion of potD (B). All samples were analyzed in triplicate and experiments were performed at least 3 times. Error bars denote standard error of the mean.