Fig 5. Differential effects on biofilm production.

Effects of potD deletion on biofilm production were determined using a biofilm assay, in which biofilm formation was assessed 24 hours after seeding 1x10⁵ pneumococci in a 24 well plate. Data is presented as OD₆₃₀. NESp PIP01 formed significantly more biofilm than WT MNZ67 (A), while T4ΔpotD produced significantly less biofilm than WT T4 (B). Samples were analyzed in triplicate and experiments were performed at least three times. Error bars denote standard error of the mean.