Osteopontin is increased in bronchoalveolar lavage (BAL) from mice
exposed to MWCNTs at 60 and 90 days. (a) mRNA gene
expression for matrix metalloproteinases (MMPs) in BAL, 60 and 90 days
after exposure to MWCNTs. (b) Osteopontin protein
concentrations in BAL, 60 and 90 days after exposure to MWCNTs.
(c) Cytospin of BAL 90 days after exposure to
MWCNTs reveals a macrophage-containing MWCNT within the cytoplasm.
(d–f) Immunohistochemistry stain of BAL for
macrophage marker MOMA (green), osteopontin
(red), and overlap (orange) in
(d) control mice, and (e) 60 days
and (f) 90 days after exposure to MWCNTs.