Figure 4.

Osteopontin is increased in bronchoalveolar lavage (BAL) from mice exposed to MWCNTs at 60 and 90 days. (a) mRNA gene expression for matrix metalloproteinases (MMPs) in BAL, 60 and 90 days after exposure to MWCNTs. (b) Osteopontin protein concentrations in BAL, 60 and 90 days after exposure to MWCNTs. (c) Cytospin of BAL 90 days after exposure to MWCNTs reveals a macrophage-containing MWCNT within the cytoplasm. (d–f) Immunohistochemistry stain of BAL for macrophage marker MOMA (green), osteopontin (red), and overlap (orange) in (d) control mice, and (e) 60 days and (f) 90 days after exposure to MWCNTs.