Figure 8.

miR-21 downregulation attenuated ALDO/SALT-mediated LV inflammation marker mRNA expression. Rats were treated with ALDO/SALT (A/S) or vehicle for 2 weeks. miR-21 antagomir (Antag) was administered starting on day 5 for 3 consecutive days. (a) LV mRNA levels of transforming growth factor β-2 (Tgfb2), (b) interleukin-1β (Il1b), and (c) monocyte chemoattractant protein-1 (MCP-1, Ccl2) were quantified by RT-qPCR. Results are expressed as mean ± standard error of the mean (n = 5). *P < 0.05 versus control (Ctrl); #P < 0.05 versus A/S. AU, arbitary unit; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.