Figure 4.

(a) Fifteen-minute excerpts from recordings of three corticotrophs treated sequentially with 2 nM AVP, 200 pM CRH, and the combination of the two, showing different levels of synergistic Ca²⁺ responses. The proportion of the AUC of the CRH plus AVP treatment and the sum of the single treatments, used as a measure of synergy, are reported under each trace. Gray lines show the theoretical sum of the responses to CRH and AVP. (b) AUC of calcium responses following subsequent exposure to CRH and AVP for the cells shown in (a). (c) Box plot showing the AUC of the calcium responses from cells treated as in (a), expressed as percentage of the sum of CRH and AVP responses (n = 51 cells from 11 experiments). ***P < 0.001, mixed effects model. The combined treatment (CRH plus AVP) was not statistically different from the sum of CRH and AVP (P = 0.91, mixed effects model).