Figure 3.
Transcripts of important inflammatory players are different between islets of WT and Gαz-KO animals, regardless of sex. T-cell markers Islets were isolated from both male and female Gαz-KO and WT NOD animals and subjected to mRNA expression analysis for (a) T-cell markers, (b) chemoattractant molecules, and (c) cytokines known to be important in T1DM pathology. Data were compared by two-way analysis of variance with Tukey post hoc test, with the exception of CD8, which was compared using Student t test; N = 3 to 5 per group. (a) P < 0.05 vs WT NOD, respective to sex; (b) P < 0.05 vs female, respective to genotype. Ct, cycle threshold; ND, not detectable.