Figure 6.

Gα_z mRNA is not expressed in splenic T-cells and does not significantly alter their type 1 T helper cell response. Splenic T-cells were harvested from female (♀) WT or Gα_z-KO mice at 17 to 19 weeks of age and separated into CD4⁺ and CD8⁺ populations by flow cytometry. (a) Gating strategy to obtain CD4⁺ and CD8⁺ T-cell populations. (b) Expression of Gnaz transcript in CD4⁺ and CD8⁺ T-cells, islets, brain, and kidney samples from WT NOD and Gα_z-KO animals. (c) Representative scatter plots for intracellular cytokine staining of TNFα and IFNγ in CD4⁺ and CD8⁺ T-cell populations stimulated with CD3ε and CD28 antibodies for 3 days. (d) Quantification of percent positive cells from data displayed in (c). Data were compared by Student t test; n = 3 to 5 per group. All error bars represent standard error of the mean. NS, not significant.