Figure 5.

Neonatal treatment with zebularine eliminates the sex difference in ERα-ir in the mPOA. (a–d) Photomicrographs of the mPOA (arrows) in a (a) vehicle-treated male (Male Veh), (b) vehicle-treated female (Female Veh), (c) zebularine-treated male (Male Zeb), and (d) zebularine-treated female (Female Zeb). (e) There was a main effect of neonatal zebularine treatment and a main effect of sex on ERα cells/mm³. Zebularine increased ERα expression, but only in males, and the sex difference was eliminated in zebularine-treated animals. n = 3 vehicle males, 4 vehicle females, 5 zebularine males, and 6 zebularine females. Scale bars = 100 µm in (a–d). *P < 0.05, **P < 0.01.