Figure 6.

There was robust ERα immunoreactivity in the (a) VMHvl and (b) mPOA of both sexes on P1. Scale bars = 50 µm. Quantification indicated no sex difference and no effect of zebularine treatment on ERα cell density (×10⁷ pixels/mm³) in either the VMHvl or mPOA. The number of animals per group were: VMHvl, n = 6 vehicle males (Male Veh), 5 vehicle females (Female Veh), 6 zebularine males (Male Zeb), and 4 zebularine females (Female Zeb); mPOA, n = 5 vehicle males, 3 vehicle females, 6 zebularine males, and 3 zebularine females.