Table 1. Raw macroscopic peak current amplitudes, shown as normalized values in Fig. 3 B, for wild-type GluN1/GluN2A or GluN1/GluN2A containing tryptophan substitutions in either GluN1 or GluN2A M4 segments.

Tryptophans in GluN1 subunit			Tryptophans in GluN2A subunit
Construct	Ipeak	n	Construct	Ipeak	n
	pA			pA
N1/N2A	−750 ± 50	18
F810W	−830 ± 290	4	I814W	−680 ± 125	4
E811W	−720 ± 95	4	D815W	−580 ± 110	4
N812W	−28 ± 6*	4	N816W	ND	9
M813W	−65 ± 9*	5	M817W	−17 ± 3*	6
A814W	−530 ± 130	4	A818W	−1,160 ± 175	5
G815W	−330 ± 90	4	G819W	−11 ± 2*	6
V816W	−250 ± 30*	4	V820W	ND	8
F817W	−1,150 ± 240	5	F821W	−370 ± 35	5
M818W	−355 ± 110	6	Y822W	−1,500 ± 120	4
L819W	−2,010 ± 210^	4	M823W	−2,090 ± 100^	5
V820W	−1,830 ± 280	4	L824W	−1,030 ± 150	4
A821W	−760 ± 110	4	A825W	−860 ± 120	4
G822W	−1,050 ± 220	4	A826W	−560 ± 65	5
G823W	−730 ± 110	4	A827W	−1,680 ± 205	5
I824W	−730 ± 150	5	M828W	−1,110 ± 150	5
V825W	−330 ± 20	4	A829W	−890 ± 130	6
A826W	−880 ± 170	4	L830W	−1,830 ± 470	5
G827W	−78 ± 8*	4	S831W	ND	9
I828W	−720 ± 90	4	L832W	−760 ± 90	5
F829W	−960 ± 130	5	I833W	−1,340 ± 100	4
L830W	−410 ± 45	4	T834W	−1,000 ± 160	4
I831W	−670 ± 60	4	F835W	−1,020 ± 190	4
F832W	−1,290 ± 220	5	I836W	−1,320 ± 160	5
I833W	−1,110 ± 130	4	W837	−750 ± 50	18
E834W	−100 ± 10*	6	E838W	−265 ± 40*	6

Values shown are mean ± SEM. n indicates the number of whole-cell recordings. Peak current amplitudes were recorded in the whole-cell mode at −70 mV (e.g., Fig. 3 A). GluN1 constructs were co-expressed with wild-type GluN2A and vice-versa. Tagged values are significantly less (*) or greater (^) than wild-type (P < 0.05, two-tailed Student’s t test, unpaired). ND, no glutamate-activated currents detected. As done previously (Salussolia et al., 2011), constructs were tested on at least two different transfection cycles with wild type tested every other transfection cycle. Constructs that showed no detectable glutamate-activated current were tested on at least one additional transfection cycle.