Table 2. Macroscopic current amplitudes for GluN1/GluN2A containing tryptophan substitutions in the VVLGAVE face in the GluN1 and GluN2A M4 segments.
| Construct | Ipeak | n | Comments | |
|---|---|---|---|---|
| pA | ||||
| N1/N2A | −750 ± 50 | 18 | ||
| V | N1(M813W)/N2A(M817W) | ND | 9 | |
| V | N1(V816W)/N2A(V820W) | NT | - | N1/N2A(V820W) showed no current |
| L | N1(V820W)/N2A(L824W) | −340 ± 35 | 4 | |
| G | N1(G823W)/N2A(A827W) | −535 ± 60 | 4 | |
| A | N1(G827W)/N2A(S831W) | NT | - | N1/N2A(S831W) showed no current |
| V | N1(L830W)/N2A(T834W) | −570 ± 140 | 4 | |
| E | N1(E834W)/N2A(E838W) | ND | 6 |
Values shown are mean ± SEM. Currents recorded as in Fig. 1. n indicates the number of whole-cell recordings. None of the constructs that showed current were significantly different from wild type (P < 0.05, two-tailed Student’s t test, unpaired). NT, not tested. ND, no glutamate-activated currents detected.