Table 5. Desensitization properties of wild-type GluN1/GluN2A or GluN1/GluN2A containing tryptophan substitutions in either GluN1 or GluN2A M4 segment.
| Tryptophans in GluN1 subunit | Tryptophans in Glu2A subunit | ||||||
|---|---|---|---|---|---|---|---|
| Construct | %des | τweighted | n | Construct | %des | τweighted | n |
| ms | ms | ||||||
| N1/N2A | 62 ± 1 | 500 ± 20 | 18 | 18 | |||
| F810W | 60 ± 3 | 510 ± 30 | 4 | I814W | 68 ± 3 | 290 ± 30 | 4 |
| E811W | 55 ± 2 | 300 ± 30 | 4 | D815W | 82 ± 3 | 230 ± 20* | 4 |
| N812W | 68 ± 2 | 88 ± 3* | 4 | N816W | – | – | |
| M813W | 77 ± 1^ | 220 ± 10* | 5 | M817W | 51 ± 1* | 990 ± 100^ | 4 |
| A814W | 64 ± 1 | 220 ± 25* | 4 | A818W | 39 ± 3 | 450 ± 70 | 4 |
| G815W | 74 ± 1^ | 48 ± 3* | 4 | G819W | 84 ± 2^ | 310 ± 40 | 5 |
| V816W | 67 ± 3 | 170 ± 20* | 4 | V820W | – | – | |
| F817W | 68 ± 2 | 220 ± 30* | 5 | F821W | 52 ± 3 | 420 ± 60 | 5 |
| M818W | 56 ± 2 | 200 ± 20* | 5 | Y822W | 48 ± 2 | 1,010 ± 145 | 4 |
| L819W | 63 ± 3 | 380 ± 60 | 4 | M823W | 93 ± 5^ | 350 ± 10 | 5 |
| V820W | 45 ± 3 | 210 ± 10* | 4 | L824W | 34 ± 4 | 1,350 ± 70^ | 4 |
| A821W | 53 ± 3 | 390 ± 50 | 4 | A825W | 77 ± 3 | 300 ± 20 | 4 |
| G822W | 68 ± 2 | 280 ± 20* | 4 | A826W | 74 ± 2 | 540 ± 30 | 5 |
| G823W | 80 ± 2^ | 310 ± 30 | 4 | A827W | 95 ± 1^ | 210 ± 10* | 5 |
| I824W | 60 ± 1 | 460 ± 30 | 5 | M828W | 64 ± 2 | 500 ± 30 | 5 |
| V825W | 47 ± 1* | 370 ± 80 | 4 | A829W | 44 ± 3 | 850 ± 160 | 5 |
| A826W | 65 ± 4 | 460 ± 20 | 4 | L830W | 52 ± 2 | 990 ± 40^ | 5 |
| G827W | 74 ± 3 | 150 ± 20* | 4 | S831W | – | – | |
| I828W | 45 ± 3 | 450 ± 40 | 4 | L832W | 54 ± 3 | 670 ± 50 | 5 |
| F829W | 59 ± 2 | 390 ± 20 | 5 | I833W | 59 ± 5 | 420 ± 30 | 4 |
| L830W | 40 ± 2* | 500 ± 70 | 4 | T834W | 55 ± 3 | 620 ± 60 | 4 |
| I831W | 63 ± 4 | 290 ± 25 | 4 | F835W | 53 ± 6 | 420 ± 50 | 4 |
| F832W | 49 ± 1* | 580 ± 40 | 5 | I836W | 50 ± 2 | 720 ± 90 | 5 |
| I833W | 67 ± 2 | 340 ± 40 | 4 | W837 | 62 ± 1 | 500 ± 20 | 18 |
| E834W | 65 ± 1 | 300 ± 20 | 6 | E838W | 68 ± 2 | 360 ± 30 | 5 |
Values shown are mean ± SEM. n indicates the number of whole-cell recordings. Tagged values are significantly less or slower (*) or greater or faster (^) than wild type (P < 0.01, t test).