Figure 5.

KLRG1⁺ ILC-2 cells from Pdcd1^−/−are efficient at clearing N. brasiliensis from Rag^−/−γc^−/− mice. WT or Pdcd1^−/− mice were infected with N. brasiliensis, and then KLRG1⁺ ILC-2 numbers in the mesenteric LNs were evaluated by flow cytometry. Representative flow plots showing GATA3⁺ ILC-2 frequency in the WT and Pdcd1^−/− (A), summary of absolute numbers of GATA3⁺ILC-2⁺ cells (B), representative flow plots showing KLRG1⁺ILC-2 subset frequency in WT and Pdcd1^−/− mice (C), and absolute numbers of KLRG1⁺ ILC-2 numbers in WT and Pdcd1^−/− mice (D). Functional cytokine expression was monitored using intracellular flow cytometry, and both frequency and absolute numbers were measured (E–G). Rag2^−/−γc^−/− mice were infected with N. brasiliensis, and then reconstituted with either WT or Pdcd1^−/− KLRG1⁺ ILC-2 cells. Worm burden (H) and eggs in the feces (I) of host mice is shown. Black circles represent WT and gray squares represent Pdcd1^−/−. Animals per cohort was n = 4–5, and data shown are mean ± SEM. Experiments were performed twice; data shown is from one repeat. A Student’s t test was performed to determine statistical significance between the various cohorts in all the panels. P ≤ 0.05 was considered significant. Significant p-values are denoted in the figures.